Microscopy:Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions. .. F
Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions.. For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.
Staining:Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions. .. F
Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions.. For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.
Enzyme-linked Immunosorbent Assay:Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions. .. F
Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions.. For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.
Control:Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions. .. F
Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions.. For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.
Inhibition:Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions. .. F
Article Title: OxLDL induces the release of IL-1β from primed human endothelial and smooth muscle cells via different caspase -1-dependent mechanisms
Article Snippet: Human coronary artery endothelial (HCAECs) and smooth muscle cells (HCASMCs) were purchased from PromoCell (Heidelberg, Germany) and maintained according to the manufacturer’s instructions.. For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.For HCAECs, lot numbers 440Z021.1 (male, 52 years old, Caucasian), 411Z027.6 (male, 58 years old, Caucasian) and 458Z035.3 (female, 61 years old, Caucasian) were used, and for HCASMCs, lot numbers 416Z048.4 (female, 63 years old, Asian), 440Z021.2 (male, 52 years old, Caucasian) and 452Z013.3 (male, 49 years old, Caucasian) were used.
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![Contractile Gene Expression Is Downregulated in Chol-Loaded hVSMCs (A, B) <t>Human</t> vascular <t>smooth</t> <t>muscle</t> <t>cells</t> (hVSMCs) were treated with cholesterol (Chol) (5 μg/mL) or 0.2% bovine serum albumin (control [CT]) for 24 hours and 48 hours and gene expression of Acta2 , Tagln , Cnn1, Myocd, and Srf were determined by quantitative polymerase chain reaction. (C) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 24 hours and protein expression of α–smooth muscle actin (α-SMA) and CNN1 were determined by Western blotting (representative blots shown). Densitometry showing the (D) α-SMA and (E) CNN1 band intensities normalized to GAPDH. For data analysis, unpaired Student’s t -testing was performed for comparing the means of 2 groups. For 2 or more independent groups, 1-way analysis of variance followed by Dunnett post hoc test was performed. A P value of ≤0.05 was considered significant. Data are presented as the mean ± SEM of 3 independent experiments, and P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5216/pmc12925216/pmc12925216__gr1.jpg)